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Changes In Hemolymph During M. sexta
Development
HINTS for SDS-PAGE Analysis of Manduca
sexta Hemolymph Proteins
- We typically pour the separating gel for the students
and let them pour the stacking gel during the lab period.
If time is an issue, then we also pour the stacking gel
(letting the students do this takes about 45
minutes).
- We have also successfully poured gels without
degassing the acrylamide solutions.
- Ethanol may be substituted for the methanol in the
destaining solutions.
- Used destain may be recycled by mixing it with
activated charcoal.
Return to the Protocol for SDS-PAGE
Analysis of Hemolymph Proteins
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