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Antibacterial response (hemolymph)

• Overview
  
  
• Bacterial treatment
  
  
• Injection
  
  
• Hemolymph collection
  
  
• Bradford assay
  
  
• Lysozyme assay
  
  
• Cecropin assay
  
  
• SDS-PAGE
  
  

Changes In Hemolymph During the Antibacterial Response of M. sexta

Protocol: Determination of Lysozyme Activity in Manduca sexta Hemolymph

Data Tables

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Hints for Lab Prep for Lysozyme Assay

Table 1 Absorbance Readings For Lysozyme Assay

 

Time	Assay Buffer	Untreated	Untreated	Untreated	Treated	Treated	Treated

 Table 2 Lysozyme Activity In Hemolymph Samples

 

SAMPLE	SLOPE	EU	EU/ml
Blank
Untreated
Untreated
Untreated
Treated
Treated
Treated

Return to the Protocol for Determination of Lysozyme Activity

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Hints for Lab Prep for Lysozyme Assay

Back to protocol for Bradford assay	On to protocol for cecropin assay

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March 1999