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Antibacterial response (hemolymph)

• Overview
  
  
• Bacterial treatment
  
  
• Injection
  
  
• Hemolymph collection
  
  
• Bradford assay
  
  
• Lysozyme assay
  
  
• Cecropin assay
  
  
• SDS-PAGE
  
  

Changes In Hemolymph During the Antibacterial Response of M. sexta

Protocol: Quantitative Determination of Proteins

Data Tables

Table 1 Absorbance Readings

sample	absorbance
10
10
10
20
20
20
40
40
40
60
60
60
80
80
80
100
100
100

Table 2 Extrapolation of Protein Concentration in Hemolymph Samples

sample	absorbance	µg protein/µl	µg protein/µl hemolymph

Return to the protocol for Protein Concentration Determination.

Back to protocol for hemolymph collection	On to protocol for lysozyme assay

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March 1999