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Equipment
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Equipment
General Laboratory Equipment
- Incubation system (a light
bulb works!)
- Flight cage, if using
adults
- An autoclave or other mechanism for sterilizing
solutions is highly desirable.
Equipment for Specific Experiments
Effect of Increased Carbon Dioxide
on Herbivory
- growth chambers for growing tobacco plants; one for
ambient CO2, one for high CO2
- leaf area meter (preferred); alternatively, access to
a photocopier
- balance capable of weighing to 0.01 g
Experiments investigating changes in hemolymph contain a
detailed equipment/supply listing for each laboratory
exercise. Some or all of the experiments may be performed to
analyze hemolymph protein, depending on time, equipment
availability, and interest.
Determination of Total Protein
Concentration
- spectrophotometer
- micropipttors
- vortexer
Determination of Lysozyme
Activity
- spectrophotometer
- stir plate
- micropipettors for measuring up to 1 ml and 0.2
ml
- pipette tips for above
- stop watch or other timing device
Determination of Cecropin
Activity
- apparatus for making approximately 3 mm holes in
agar. Although units are commercially available, this can
simply be a large end of a Pasteur pipette that has been
broken and attached to a vacuum apparatus.
SDS-PAGE Analysis
- units for protein electrophoresis, preferably
mini-units (Mini
Protean II from BioRad
are excellent, catalog number 165-2940). The system
should include glass plates, spacers, combs,
electrophoresis gel box with lid, electrical cables.
- electrophoresis power supply
- micropipettors
- vacuum apparatus (preferred but can do without)
- rotating shaker
- gel dryer (preferred but can do without)
Disclaimer: Supplier links are provided
for your convenience only; these are not paid
endorsements.
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