The plasmids we are using have known restriction enzyme cutting sites. Each restriction enzyme recognizes a specific, short sequence of bases and cuts the DNA, leaving "sticky ends." See the lab manual for details.

The restriction enzymes we are using are called "BamHI" (pronounced "bam H one") (from Bacillus amyloliquefaciens H) and "HindIII" (pronounced "hin dee three") (from Haemophilus influenzae Rd). Each of these enzymes has one cutting site on each plasmid. These sites are highlighted in orange on the pKAN and pAMP plasmid sequences.

The restriction enzyme maps of these plasmids are shown below:

The distances between restriction enzyme sites are shown in base pairs. Click on each image to see the plasmid digestion. A new window will pop up; in some browsers, this window may be behind your current window. Click the "Reload" or "Refresh" button in the new window to watch the animation again.